DNA duplex stability refers to the strength and stability of the double helix structure of DNA. The stability of the DNA duplex is crucial for the proper functioning of genetic information transfer and gene expression. There are various synonyms used to describe DNA duplex stability, such as hybridization strength, melting temperature, thermal stability, annealing efficiency, and duplex formation. These terms describe the ability of complementary DNA strands to bind and form a stable duplex structure. Furthermore, DNA hybridization assays are commonly used to determine the duplex stability of DNA and are crucial in research and diagnostic applications where DNA interactions are required.